Inclusion of MERS-spike protein ELISA in algorithm to determine serologic evidence of MERS-CoV infection.
Identifieur interne : 000939 ( Main/Exploration ); précédent : 000938; suivant : 000940Inclusion of MERS-spike protein ELISA in algorithm to determine serologic evidence of MERS-CoV infection.
Auteurs : Suvang Trivedi [États-Unis] ; Congrong Miao [Géorgie (pays)] ; Mohammad M. Al-Abdallat [Jordanie] ; Aktham Haddadin [Jordanie] ; Sultan Alqasrawi [Jordanie] ; Ibrahim Iblan [Jordanie] ; Mohannad A. Nsour [Jordanie] ; Tarek Alsanouri [Jordanie] ; Sami S. Ali [Jordanie] ; Brian Rha [Géorgie (pays)] ; Susan I. Gerber [Géorgie (pays)] ; Daniel C. Payne [Géorgie (pays)] ; Azaibi Tamin [Géorgie (pays)] ; Natalie J. Thornburg [Géorgie (pays)]Source :
- Journal of medical virology [ 1096-9071 ] ; 2018.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Infections à coronavirus.
- immunologie : Coronavirus du syndrome respiratoire du Moyen-Orient, Glycoprotéine de spicule des coronavirus.
- sang : Anticorps antiviraux.
- Algorithmes, Humains, Test ELISA, Tests sérologiques.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antibodies, Viral.
- diagnosis : Coronavirus Infections.
- immunology : Middle East Respiratory Syndrome Coronavirus, Spike Glycoprotein, Coronavirus.
- methods : Enzyme-Linked Immunosorbent Assay, Serologic Tests.
- Algorithms, Humans.
Abstract
The Centers for Disease Control and Prevention (CDC) algorithm for detecting presence of serum antibodies against Middle East Respiratory Syndrome coronavirus (MERS-CoV) in subjects with potential infections with the virus has included screening by indirect ELISA against recombinant nucleocapsid (N) protein and confirmation by immunofluorescent staining of infected monolayers and/or microneutralization titration. Other international groups include indirect ELISA assays using the spike (S) protein, as part of their serological determinations. In the current study, we describe development and validation of an indirect MERS-CoV S ELISA to be used as part of our serological determination for evidence of previous exposure to the virus.
DOI: 10.1002/jmv.24948
PubMed: 28906003
Affiliations:
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Ctr. for Immunization and Respiratory Diseases, CDC, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Tamin, Azaibi" sort="Tamin, Azaibi" uniqKey="Tamin A" first="Azaibi" last="Tamin">Azaibi Tamin</name><affiliation wicri:level="1"><nlm:affiliation>Respiratory Viruses Branch, Div. of Viral Diseases, Natl. Ctr. for Immunization and Respiratory Diseases, CDC, Atlanta, Georgia.</nlm:affiliation><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Respiratory Viruses Branch, Div. of Viral Diseases, Natl. Ctr. for Immunization and Respiratory Diseases, CDC, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Thornburg, Natalie J" sort="Thornburg, Natalie J" uniqKey="Thornburg N" first="Natalie J" last="Thornburg">Natalie J. Thornburg</name><affiliation wicri:level="1"><nlm:affiliation>Respiratory Viruses Branch, Div. of Viral Diseases, Natl. Ctr. for Immunization and Respiratory Diseases, CDC, Atlanta, Georgia.</nlm:affiliation><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Respiratory Viruses Branch, Div. of Viral Diseases, Natl. Ctr. for Immunization and Respiratory Diseases, CDC, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of medical virology</title><idno type="eISSN">1096-9071</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Algorithms</term><term>Antibodies, Viral (blood)</term><term>Coronavirus Infections (diagnosis)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Humans</term><term>Middle East Respiratory Syndrome Coronavirus (immunology)</term><term>Serologic Tests (methods)</term><term>Spike Glycoprotein, Coronavirus (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Algorithmes</term><term>Anticorps antiviraux (sang)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Glycoprotéine de spicule des coronavirus (immunologie)</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Test ELISA ()</term><term>Tests sérologiques ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Glycoprotéine de spicule des coronavirus</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Serologic Tests</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Algorithms</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Algorithmes</term><term>Humains</term><term>Test ELISA</term><term>Tests sérologiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Centers for Disease Control and Prevention (CDC) algorithm for detecting presence of serum antibodies against Middle East Respiratory Syndrome coronavirus (MERS-CoV) in subjects with potential infections with the virus has included screening by indirect ELISA against recombinant nucleocapsid (N) protein and confirmation by immunofluorescent staining of infected monolayers and/or microneutralization titration. Other international groups include indirect ELISA assays using the spike (S) protein, as part of their serological determinations. In the current study, we describe development and validation of an indirect MERS-CoV S ELISA to be used as part of our serological determination for evidence of previous exposure to the virus.</div></front></TEI><affiliations><list><country><li>Géorgie (pays)</li><li>Jordanie</li><li>États-Unis</li></country><region><li>Tennessee</li></region></list><tree><country name="États-Unis"><region name="Tennessee"><name sortKey="Trivedi, Suvang" sort="Trivedi, Suvang" uniqKey="Trivedi S" first="Suvang" last="Trivedi">Suvang Trivedi</name></region></country><country name="Géorgie (pays)"><noRegion><name sortKey="Miao, Congrong" sort="Miao, Congrong" uniqKey="Miao C" first="Congrong" last="Miao">Congrong Miao</name></noRegion><name sortKey="Gerber, Susan I" sort="Gerber, Susan I" uniqKey="Gerber S" first="Susan I" last="Gerber">Susan I. Gerber</name><name sortKey="Payne, Daniel C" sort="Payne, Daniel C" uniqKey="Payne D" first="Daniel C" last="Payne">Daniel C. Payne</name><name sortKey="Rha, Brian" sort="Rha, Brian" uniqKey="Rha B" first="Brian" last="Rha">Brian Rha</name><name sortKey="Tamin, Azaibi" sort="Tamin, Azaibi" uniqKey="Tamin A" first="Azaibi" last="Tamin">Azaibi Tamin</name><name sortKey="Thornburg, Natalie J" sort="Thornburg, Natalie J" uniqKey="Thornburg N" first="Natalie J" last="Thornburg">Natalie J. Thornburg</name></country><country name="Jordanie"><noRegion><name sortKey="Al Abdallat, Mohammad M" sort="Al Abdallat, Mohammad M" uniqKey="Al Abdallat M" first="Mohammad M" last="Al-Abdallat">Mohammad M. Al-Abdallat</name></noRegion><name sortKey="Ali, Sami S" sort="Ali, Sami S" uniqKey="Ali S" first="Sami S" last="Ali">Sami S. Ali</name><name sortKey="Alqasrawi, Sultan" sort="Alqasrawi, Sultan" uniqKey="Alqasrawi S" first="Sultan" last="Alqasrawi">Sultan Alqasrawi</name><name sortKey="Alsanouri, Tarek" sort="Alsanouri, Tarek" uniqKey="Alsanouri T" first="Tarek" last="Alsanouri">Tarek Alsanouri</name><name sortKey="Haddadin, Aktham" sort="Haddadin, Aktham" uniqKey="Haddadin A" first="Aktham" last="Haddadin">Aktham Haddadin</name><name sortKey="Iblan, Ibrahim" sort="Iblan, Ibrahim" uniqKey="Iblan I" first="Ibrahim" last="Iblan">Ibrahim Iblan</name><name sortKey="Nsour, Mohannad A" sort="Nsour, Mohannad A" uniqKey="Nsour M" first="Mohannad A" last="Nsour">Mohannad A. Nsour</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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